Screening method for ligands with class II P13 kinase modulating activity

ABSTRACT

A novel human class II PI3 kinase including the sequence of the isolated nucleic acid molecule which encodes the kinase and the encoded amino acid sequence. The new human PI3 kinase is termed PI3K-C2α and has unique biochemical properties that characterize it and distinguish it from previously known PI3 kinases. These include, among other things, resistance to the PI3 kinase inhibitors Wortmannin and LY294000, the lack of a p85 binding site, a divergent amino terminus, and the absence of a polyproline motif which is typical of previously known type II PI3 kinases.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a division of co-pending U.S. patent application Ser. No. 09/355,160, now U.S. Pat. No. 6,436,671 filed on Oct. 1, 1999, which is the U.S. national stage of international patent application no. PCT/GB98/00244, filed Jan. 27, 1998, designating the United States of America. Priority is claimed based on United Kingdom patent application Ser. No. GB 9701652.1, filed Jan. 28, 1997.

BACKGROUND OF THE INVENTION

The invention relates to a novel class II PI3 kinase, termed PI3K-C2α, and in particular the isolation thereof having regard to its relevant sequence structure and/or biochemical characteristics; means used in the isolation or production thereof; antibodies adapted to bind thereto; and assay kits relating thereto.

Phosphoinositides have been implicated in a variety of cellular processes as diverse as vacuolar protein sorting (1,2), cytoskeletal remodelling (3) and mediating intracellular signalling events through which growth factors, hormones and neurotransmitters exert their physiological effects on cellular activity, proliferation and differentiation (4,5,6).

Recently a family of proteins have been cloned and characterised and shown to be enzymes catalysing the addition of phosphate to inositol. Eukaryotic cells contain a variety of inositol derivatives phosphorylated to different extents. PtdIns(3)P is constitutively present in eukaryotic cells and its levels are constant upon extracellular stimulation. PtdIns(3,4)P₂ and PtdIns(3,4,5)P₃ (7,8) are virtually absent in resting cells but are rapidly induced upon stimulation with a variety of ligands. The enzymes catalysing these reactions are phosphoinositide lipid kinases (hereinafter called PI3 kinases). A brief overview of the current data in relation to PI3 kinases classifies these enzymes into three distinct groups being designated to an individual class by their in vitro substrate specificity, biochemical characteristics and, in examples where a definitive function has been assigned, the nature of the biochemical activity regulated by the specific kinase.

PI3 kinase class 1 polypeptides have a broad spectrum activity, phosphorylating inositol lipids PtdIns, PtdIns(4)P and PtdIns(4, 5)P₂. Class I kinases are subdivided into Class IA and IB. Class IA polypeptides include p110α (9), p110β (10) and p110δ (11) which interact physically with the adaptor sub-unit protein p85. Moreover, p110α p100 have a broad distribution in terms of expression pattern. p110δ expression seems to be restricted to white blood cells. Class 1B includes p110γ (12) which functions independently of p85. Although each of these Class 1 kinases catalyse phosphate addition to inositol lipid, the mechanism via which these enzymes are activated and regulated is achieved by different molecular mechanisms.

Class II PI3 kinases have a restricted substrate specificity phosphorylating PtdIns and PtdIns(4)P but not PtdIns(4,5) P₂. Each of the kinases of this class are characterised by a conserved C2 domain in the carboxyl terminal region of the protein. The presence of conserved motifs within the C2 domain indicates that this region may confer regulation via calcium and/or phospholipid. A comparison of the murine and Drosophila class II kinases mp170 and PI3K_(—)68D respectively reveals a high degree of homology in the kinase domain of these proteins. Significant divergence occurs at the amino terminal regions of these polypeptides suggesting that adaptor proteins interacting with these variable domains may regulate kinase activity. Class II PI3 kinases do not interact with p85.

The third class of PI3 kinase, class III PI3 Kinase, is related to the S.cerevisiae gene Vps34 (1). This kinase was originally isolated as a gene involved in regulating vesicle mediated membrane-trafficking in yeast. The human homologue of Vps34 is complexed with a ser/thr kinase called Vps15p (14,15). Of the three classes of PI3 kinase this has the most restricted substrate specificity being strictly limited to PtdIns.

SUMMARY OF THE INVENTION

A novel human class II PI3 kinase is herein described and termed human PI3K-C2α. It is characterised as a class II kinase due to the presence of a conserved C2 domain found in murine and Drosophila class II PI3 kinases (FIG. 2), its apparent lack of a p85 binding site and a substrate specificity limited to PtdIns and PtdIns (4) P (FIG. 4). The polypeptide is unique in that this is the first human class II kinase to be described. It has significant divergence in the amino terminal region of the protein when compared to the mouse homologue of human PI3K-C2α (16). It is also, surprisingly, the first PI3 kinase to be isolated that has resistance to PI3 kinase inhibitors Wortmannin and LY294002 (FIG. 5).

The use of selected inhibitors has proved extremely useful in analysing intracellular signal transduction cascades. Inhibitors used at low concentrations probably result in the modification of a single protein's function thereby allowing the dissection of single transduction pathways. A good example of this is the use of Pertussis toxin which is a cell permeant agent (17). The agent undergoes endocytosis into intact cells and results in the ADP-ribosylation of specific GTP-binding or G-proteins. This modification uncouples these G-proteins from their receptors therefore interfering with the cell's response to receptor stimuli. Wortmannin is another cell permeant inhibitor (18,19). It is a fungal metabolite and has been shown to have in vivo anti-inflammatory or immunosuppressive effects in animal models. Wortmannin was first shown to inhibit cellular responses to receptor stimulation in neutrophils. The drug inhibited the respiratory burst induced by ligands such as N-formyl-Met-Leu-Phe,(fMLP),C5a,leukotriene B4 or platelet-activating factors. Importantly, Wortmannin failed to inhibit cellular response to TPA suggesting differential responses to the drug. In particular, the fact that the stimulation of calcium mobilising receptors is resistant to Wortmannin suggests that intracellular signalling initiated by these receptors is controlled by a quite separate kinase cascade.

The identification of PI3 kinases as the target for Wortmannin came from in vitro metabolic labelling of guinea pig neutrophils with ³²p to monitor the uptake of phosphate into phospholipids in the presence of specific kinase inhibitors (20). In control experiments stimulation of fMLP receptors resulted in ³²p labelling of phosphatidic acid and PtdIns(3,4,5)P₃. The presence of Pertussis toxin had no effect on the phosphorylation of these phospholipids. However, pre-incubation of leucocytes with Wortmannin resulted in inhibition of ³²P incorporation into only PtdIns(3,4,5)P₃. Since this is the product of PI3 kinase catalysed reactions it seems likely that Wortmannin was specifically targeting PI3 kinase. Subsequently, Wortmannin has been shown to block a number of physiological processes including many insulin stimulated actions that would result in enhanced glucose utilisation (21). Wortmannin has proved to be an effective inhibitor of mammalian PI3 kinases. To date, no human PI3 kinase has been cloned and shown to be resistant to this agent.

The isolation and sequencing of an as yet unidentified human PI3 kinase that has significant homology to previously identified murine and Drosophila class II kinases is described (16,22,23). Comparison of the optimal alignment of these aforementioned proteins shows the human protein to be 32.5% homologous with sequences of the Drosophila PI3K_(—)68D and cpk proteins and 90.8% and 90.2% with the murine proteins mp107 and mcpk, FIG. 2. The carboxyl terminal region of the aforementioned proteins have increased homology due to the presence of a conserved C2 domain. This domain is characterised by the presence of motifs likely to be involved in the modulation of kinase activity by calcium and/or phospholipid.

The amino terminal region of human PI3K-C2α is extended by 176 amino acid residues which are lacking in the murine sequence of mp170. The murine mcpk protein has a 28 amino acid residue deletion in this region that is absent from human PI3K-C2α and a mp170. This divergence in sequence may be explained by the presence of the unique binding sites for adaptor proteins that regulate kinase activity. This is supported by the lack of an apparent p85 binding motif.

Furthermore, immunofluoresence experiments using a monoclonal antibody to the amino terminal glu-tag of PI3K-C2α reveals a punctate cytoplasmic distribution in Cos cells expressing the recombinant glu-tagged PI3K-C2α protein, FIG. 9. Moreover subcellular fractionation of Monkey Kidney Cos cells shows that epitope tagged PI3K-C2α is tightly associated with phospholipid containing membranes. The nature of this interaction is currently unknown but may be mediated via phospholipid interaction with the C2 domain or alternatively through binding to an additional polypeptide localized to microsomes and plasma membranes.

The recombinantly produced PI3K-2Cα kinase shows increased resistance to the general PI3 kinanse inhibitors Wortmannin and LY294002, (FIG. 5) To date, aall clones human PI3 kinases have been found to be sensitive to these inhibitors. Studies of the Wortmannin sensitive class I PI3 kinanse p110α identifies residue lys-802 as being the sit of Wortmannin covalent inactivation of kinase activity, a residue near or at the active site and involved in phosphate transfer. This residue is present in all PI3 kinase family members and also in PI3K-2Cα, therefore the resistance to Wortmannin by PI3K-2Cα is not apparent.

Additionally it will be apparent that alternate methods for use in modulating the activity of PI3K-2Cα exist.

For example, having regard to the sequence data provided herein it is possible to provide antisense material which prevents the expression of PI3K-2Cα.

As mentioned above, the invention embraces antisense oligonucleotides that selectively bind to a nucleic acid molecule encoding a PI3K-2Cα protein, to decrease transcription and/or translation of PI3K-2Cα genes. This is desirable in virtually any medical condition wherein a reduction in PI3K-2Cα gene product expression is desirable, including to reduce any aspect of a tumor cell phenotype attributable to PI3K-2Cα gene expression. Antisense molecules, in this manner, can be used to slow down or arrest such aspects of a tumor cell phenotype.

As used herein, the term “antisense oligonucleotide” or “antisense” describes an oligonucleotide that is an oligoribonucleotide, oligodeoxyribonucleotide, modified oligoribonucleotide, or modified oligodeoxyribonucleotide with hybridizes under physiological conditions to DNA comprising a particular gene or to an mRNA transcript of that gene and thereby, inhibits the transcription of that gene and/or the translation of that mRNA. The antisense molecules are designed so as to interfere with transcription or translation of a target gene upon hybridization with the target gene. Those skilled in the art will recognize that the exact length of the antisense oligonucleotide and its degree of complementarity with its target will depend upon the specific target selected, including the sequence of the target and the particular bases which comprise that sequence. It is preferred that the antisense oligonucleotide be constructed and arranged so as to bind selectively with the target under physilogical conditions, i.e., to hybridize substantially more to the target sequence than to any other sequence in the target cell under physiological conditions. Based upon the DNA sequence presented in FIG. 1 or upon allelic or homologous genomic and/or DNA sequences, one of skill in the art can easily choose and synthesize any of a number of appropriate antisense molecules for use in accordance with the present invention. In order to be sufficiently selective and potent for inhibition, such antisense oligonucleotides should comprise at least 7 (Wagner et al., Nature Biotechnology 14:840-844, 1996) and more preferably, at least 15 consecutive bases which are complementary to the target. Most preferably, the antisense oligonucleotides comprise a complementary sequence of 20-30 bases. Although oligonucleotides may be chosen which are antisense to any region of the gene or mRNA transcripts, in preferred embodiments the antisense oligonucleotides correspond to N-terminal or 5′ upstream sites such as translation initiation, transcription initiation or promoter sites. In addition, 3′-untranslated regions may be targeted. Targeting to mRNA splicing sites has also been used in the art but may be less preferred if alternative mRNA splicing occurs. In addition, the antisense is targeted, preferably, to sites in which mRNA secondary structure is not expected (see, e.g., Sainio et al., Cell Mol. Neurobiol. 14(5):439-457. 1994) and at which proteins are not expected to bind. Finally, although FIG. 1 discloses cDNA sequence, one of ordinary skill in the art may easily derive the genomic DNA corresponding to the cDNA of FIG. 1. Thus, the present invention also provides for antisense oligonucleotides which are complementary to the genomic DNA corresponding to FIG. 1. Similarly, antisense to allelic or homologous DNAs and genomic DNAs are enabled without undue experimentation.

In one set of embodiments, the antisense oligonucleotides of the invention may be composed of “natural” deoxyribonucleotides, ribonucleotides, or any combination thereof. That is, the 5′ end of one native nucleotide and the 3′ end of another native nucleotide may be covalently linked, as in natural systems, via a phosphodiester internucleoside linkage. These oligonucleotides may be prepared by art recognized methods which may be carried out manually or by an automated synthesizer. They also may be produced recombinantly by vectors.

In preferred embodiments, however, the antisense oligonucleotides of the invention also may include “modified” oligonucleotides. That is, the oligonucleotides may be modified in a number of ways which do not prevent them from hybridizing to their target but which enhance their stability or targeting or which otherwise enhance their therapeutic effectiveness.

The term “modified oligonucleotide” as used herein describes an oligonucleotide in which (1) at least two of its nucleotides are covalently linked via a synthetic intemucleoside linkage (i.e., a linkage other than a phosphodiester linkage between the 5′ end of one nucleotide and the 3′ end of another nucleotide) and/or (2) a chemical group not normally associated with nucleic acids has been covalently attached to the oligonucleotide. Preferred synthetic internucleoside linkages are phosphorothioates, alkylphosphonates, phosphorodithioates, phosphate esters, alkylphosphonothioates, phosphoramidates, carbamates, phosphate triesters, acetamidates, peptides, and carboxymethyl esters.

The term “modified oligonucleotide” also encompasses oligonucleotides with a covalently modified base and/or sugar. For example, modified oligonucleotides include oligonucleotides having backbone sugars which are covalently attached to low molecular weight organic groups other than a hydroxyl group at the 3′ position and other than a phosphate group at the 5′ position. Thus modified oligonucleotides may include a 2′-O-alkylated ribose group. In addition, modified oligonucleotides may include sugars such as arabinose instead of ribose. Modified oligonucleotides also can include base analogs such as C-5 propyne modified bases (Wagner et al., Nature Biotechnology 14:840-844, 1996). The present invention, thus, contemplates pharmaceutical preparations containing modified antisense molecules that are complementary to and hybridizable with, under physiological conditions, nucleic acids encoding PI3K-C2α proteins, together with pharmaceutically acceptable carriers.

Antisense oligonucleotides may be administered as part of a pharmaceutical composition. Such a pharmaceutical composition may include the antisense oligonucleotides in combination with any standard physiologically and/or pharmaceutically acceptable carriers which are known in the art. The compositions should be sterile and contain a therapeutically effective amount of the antisense oligonucleotides in a unit of weight or volume suitable for administration to a patient. The term “pharmaceutically acceptable” means a non-toxic material that does not interfere with the effectiveness of the biological activity of the active ingredients. The term “physiologically acceptable” refers to a non-toxic material that is compatible with a biological system such as a cell, cell culture, tissue, or organism. The characteristics of the carrier will depend on the route of administration. Physiologically and pharmaceutically acceptable carriers include diluents, fillers, salts, buffers, stabilizers, solubilizers, and other materials which are well known in the art.

It follows from the foregoing description that it is an object of this invention to isolate the nucleic acid sequence and derived amino acid sequence of the first human class II PI3 kinase.

It is a further object of this invention to recombinantly manufacture human PI3K-C2α kinase.

It is a further object of this invention to use computer assisted three dimensional reconstruction of human PI3K-C2α kinase protein to facilitate the identification of agonists or antagonists of PI3K-C2α.

It is a yet further object to use said three dimensional reconstruction to design ligands that may modulate the biochemical activity of human PI3-C2α kinase.

It is yet a further object of this invention to manufacture an antibody, preferably monoclonal, to PI3K-C2α, and more preferably further still to manufacture a humanised antibody to PI3K-C2α.

It is yet a further object of this invention that the monoclonal antibody be specific to class II PI3 kinases.

It is yet a further object of this invention to provide an assay kit for use in identifying said protein.

According to the first aspect of the invention there is provided the isolated nucleic acid molecule and/or corresponding protein of human PI3K-C2α as shown in FIG. 1, including any deletions, additions or substitutions, or a complementary sequence thereto.

In a preferred embodiment of the invention said complementary sequence binds to said nucleic acid sequence under stringent conditions. Preferably, these conditions include 0.5M sodium phosphate, pH7.2, 7% SDS, 1 mM EDTA at 65° C.

In yet a further preferred embodiment of the invention said deletions, additions or substitutions do not alter the function of the protein or indeed do not alter the function in a deleterious manner thus, in one example, a nucleic acid molecule may be provided that binds to the relevant gene to prevent expression of PI3K-C2α.

In yet a further preferred embodiment of the invention said deletions, additions or substitutions do alter the function of the protein. Thus, in one example, a mutation in PI3K-C2α could produce a dominant negative mutation that results in the inactivation of endogenous PI3K-C2α in transfected or transformed cells.

According to a second aspect of the invention there is provided an antibody, or at least an effective part thereof, which binds at least with a selective part of said human PI3K-C2α. Ideally said effective part comprises FAb fragments. Ideally said antibody is polyclonal or monoclonal and ideally further still said antibody is specific to class II PI3 Kinases. Ideally said antibody is humanised by recombinant methods to contain the variable region of said antibody with an invariant or constant region of a human antibody.

Ideally said antibody is provided with a marker including a conventional label or tag, for example a radioactive and/or fluorescent and/or epitope label or tag.

More ideally still said humanized monoclonal antibody to class II PI3- kinases is produced as a fusion polypeptide in an expression vector suitably adapted for transfection or transformation of prokaryotic or eukaryotic cells.

According to a yet further aspect of the invention there is provided a construct including all, or part of, the said nucleic acid molecule of the invention.

In a preferred embodiment of the invention said construct is adapted to be a cloning vehicle for human PI3K-C2α.

In a preferred embodiment of the invention said cloning vehicle may be used for expression of the protein sequence of human PI3K-C2α in transformed or transfected prokaryotic and eukaryotic cells. Preferably said cloning vehicle is adapted for use in E. coli, mammalian, insect, amphibian or fungal cells.

In a preferred embodiment of the invention said construct includes nucleic acid encoding at least a part of human PI3K-C2α which is functionally linked to a promoter so as to provide for either constitutive or inducible expression of human PI3K-C2α kinase. Preferably further still said construct is adapted for cell/tissue specific expression of PI3K-C2α.

According to a yet further aspect of the invention there is provided cells transformed or transfected with the cloning vehicle of the invention and so including the nucleic acid sequence molecule encoding human PI3K-C2α or a part thereof.

According to a yet further aspect of the invention there is provided recombinantly manufactured human PI3K-C2α.

According to a yet further aspect of the invention there is provided human PI3K-C2α kinases or a fraction thereof, which is characterised by insensitivity to the PI3 kinase(s) inhibitor Wortmannin and/or LY294002.

In a further preferred embodiment of this aspect of the invention said protein is also, or alternatively, characterised by the absence of a p85 binding site.

In a further preferred embodiment of this aspect of the invention said protein is also, or alternatively, characterised by the absence of a type II polyproline motif present in Drosophilia P13K_(—)68D and cpk.

In a yet a further preferred embodiment of this aspect of the invention said protein is also, or alternatively, characterised by divergent amino terminal region when compared to other class II PI3 kinases.

In a yet a further preferred embodiment of this aspect of the invention said protein is characterised by a molecular weight of 190 Kda.

In yet a further preferred embodiment of this aspect of the invention said protein is also, or alternatively, characterised by an in vitro substrate specificity that is restricted to PtdIns, PtdIns(4)P or at least PtdIns, PtdIns(4)P.

In any of the above embodiments of the invention said fraction ideally comprises the full length amino acid sequence or truncations thereof.

According to a further aspect of the invention there is provided the isolated genomic DNA and/or cDNA of human PI3K-C2α kinase according to any previous aspect of the invention.

According to a yet further aspect of the invention there is provided a process for the isolation of human PI3K-C2α kinase which process includes a PCR reaction.

In a preferred process of the invention said PCR reaction comprises use of at least two primers adapted to bind to at least one selected part of nucleic acid of human PI3K-C2α; and further said process also comprises the provision of conditions for amplifying at least one selected part of said nucleic acid using said primers; and further, following amplification, said process comprises the purification of the amplified product(s); and, optionally, isolation of at least one amplified fragment.

In a preferred process of the invention said primers are designed to selectively bind to the nucleic acid of class II kinases and ideally class II human kinases.

In yet a further preferred embodiment of the invention said primers are as follows: sense: 5′GGNGA T/C GA T/C T/C T A/G CGNCA A/G GA3′ (SEQ ID NO:4) antisense: 5′A/G AA A/G TGICC A/G AA A/G TC A/G/T AT A/G TG A/G TG A/G AA3′ (SEQ ID NO:5)

In yet a further preferred embodiment of the invention said primers include any one or more deletions, additions or substitutions which do not deleteriously affect the functional effectiveness of the primers.

According to a yet further aspect of the invention there is provided the use of any one of more of the aforementioned primers to isolate class II PI3 kinases and ideally human PI3K-C2α.

According to a yet further aspect of the invention there is provided the use of any one or more of the aforementioned primers to isolate human PI3 kinases and ideally human PI3K-C2α.

According to a yet further aspect of the invention there is provided an agent which is adapted to interact with the amino terminal region, or any part thereof, of human PI3K-C2α kinase.

According to a yet further aspect of the invention there is provided an assay kit for identifying human PI3K-C2α either intracellularly or in a cellular extract.

In a preferred embodiment of the invention said assay kit is a diagnostic kit.

Preferably said assay kit, or said diagnostic kit makes use of any one or more of the following techniques: western blotting; immunoprecipitation; immunofluorescence on whole cells or tissue sections; or quantitative ELISA reactions.

According to a yet further aspect of the invention there is provided a method for identifying agonists or antagonists effective at enhancing or blocking the activity of the polypeptide of the invention which comprises screening candidate molecules for such activity using the polypeptide, or fragments thereof the invention.

Ideally, screening may involve artificial techniques such as computer-aided techniques or conventional laboratory techniques.

Alternatively, the method of the invention may involve competitive binding assays in order to identify agents that selectively and ideally irreversibly bind to the polypeptide of the invention.

According to a yet further aspect of the invention there is provided a pharmaceutical or veterinary composition comprising an agent effective at enhancing or blocking the activity of the polypeptide of the invention which has been formulated for pharmaceutical or veterinary use and which optionally also includes a dilutant, carrier or excipient and/or is in unit dosage form.

According to a yet further aspect of the invention there is provided antisense oligonucleotide or a modified antisense oligonucleotide as hereindescribed adapted to hybridise to at least part of the nucleic acid molecule shown in FIG. 1.

Thus, in general terms the invention describes the nucleic acid sequence and derived amino acid sequence of a novel human class II PI3 kinase, human PI3K-C2α, which has novel features in relation to sequence, biochemistry and responses to well characterised inhibitors of PI3 kinases.

BRIEF DESCRIPTION OF THE DRAWINGS

The invention will be described in further detail with reference to illustrative preferred embodiments and experimental results depicted in the accompanying drawings in which:

FIG. 1 represents the complete cDNA sequence (SEQ ID NO: 1) and amino acid sequence (SEQ ID NO:2) of PI3K-C2α;

FIGS. 2a, 2 b and 2 c show a comparison of the conserved domains found in type II PI3 kinases and in particular the kinase domains and C2-domains;

FIG. 3 represents a northern blot the distribution of mRNA encoding PI3K-C2α in various tissues;

FIGS. 4a and 4 b represent the in vitro substrate specificity of PI3K-C2α in the presence of Mg²⁺ and Mn²⁺, p110α is included as a positive control;

FIG. 5 represents the resistance of recombinant PI3K-C2α to the PI3-kinase inhibitors Wortmannin and LY 294002, (p110α=•, recombinant PI3K-C2α=o);

FIG. 6 is a diagrammatic representation of the sub-cellular fractionation of Cos 7 cells into various membrane fractions;

FIG. 7 represents a western blot of cell lysates prepared from various cell-lines and probed with antisera to PI3K-C2α;

FIG. 8 represents a western blot of sub-cellular fractions prepared from Cos 7 cells and probed with antisera to PI3K-C2α; and

FIG. 9 represents an immunofluoresence image of Cos7 cells expressing recombinant glu-tagged PI3K-C2α.

MATERIALS AND METHODS Identification of PI3K-C2α cDNA

Poly A+RNA was isolated from U937 cells using oligo(dT) cellulose (Stratagene) and used to synthesise first strand cDNA with AMV reverse transcriptase (Pharmacia). PCR reactions were then performed using Taq DNA polymerase (Life Technologies), first strand cDNA, 4 mM Mg2+ and 1 μM primers. The nucleotide sequences of the primers used were sense—GGNGA T/C GA T/C T/C T A/G CGNCA A/G GA (SEQ ID NO:4) [GDDLRQD/E (SEQ ID NO:9)] and antisense—A/G AA A/G TGICC A/G AA A/G TC A/G/T AT A/G TG A/G TG A/G AA (SEQ ID NO:5) [FHIDFGHF (SEQ ID NO:10)]. To facilitate subsequent cloning, an EcoRI restriction site was incorporated into the 5′ nd of each primer. The conditions used were 30 cycles of 94° C. for 30s, 56° C. for 30s, and 72° C. for 30s. After sub cloning into a Bluescript-SK vector, individual clones were selected and sequenced (ABI).

Isolation of PI3K-C2α cDNA

The PCR fragment was excised from the vector, labelled with ³² P dCTP using random primers (Amersham) and used to screen a 1 λzap U937 cDNA library (Stratagene). Approximately 3×10⁶ plaques were plated and transferred onto Hybond-N filters (Amersham). These were hybridised with the probe for 16 hours at 65° C. in 0.5M sodium phosphate pH7.2, 7% SDS and 1 mM EDTA. After this time, the filters were washed twice with 0.5x SSC/0.1% SDS for 20 minutes at 60° C. and positive clones identified by autoradiography. After three rounds of screening, clones were plaque purified and the cDNA insert in Bluescript-SK was rescued using ExAssist helper phage (Stratagene). The nucleotide sequence of the longest clone was determined using both Taq di deoxy terminator cycle sequencing (ABI) and di deoxy chain termination using T7 DNA polymerase and a ³⁵S thio dATP (Lark Sequencing Technologies).

The 5′ end of the eDNA was obtained by rapid amplification of cDNA ends. cDNA was synthesised (Life Technologies) using primers complementary to the original cDNA sequence [CTCTTCCTCATGGTCTAATACCTCCAC (SEQ ID NO:3) and TATCTCCAAATCAGTCCTTGCTTTCCC (SEQ ID NO:6)] from poly A+ mRNA obtained from U937 cells. After sequencing, this fragment was ligated onto the parent cDNA in a pBK-CMV vector (Stratagene) to produce the full length PI3K-C2α cDNA.

Northern Blot Analysis

Multiple human tissue poly A+ mRNA blots (Clontech) were hybridised with a 1.46 kb cDNA fragment corresponding to nucleotides 789-2249 of the PI3K-C2α coding sequence (SEQ ID NO:1). The probe was labelled using [α ³²p] dCTP and random primers (Amersham). Hybridizations were performed in Express Hyb (Clontech) at 65° C. for 16 hours. The blots were washed twice with 2xSSC, 0.05%SDS at room temperature and twice in 1xSSC, 0.1%SDS at 50° C. Bands were visualised by autoradiography.

Plasmid Constructs and Expression of Recombinant Protein in Sf9 Cells

PI3K-C2α cDNA corresponding to amino acid residues 148-1686 of SEQ ID NO:2 was ligated in the pAcGex vector (44). Recombinant DNA (2 μg) was transfected into Sf9 cells with 0.25 μg BaculoGold DNA (Pharmingen) using Lipofectin (Gibco). Infections and amplifications (6×10⁵ cells/ml) of the viral stocks were performed for 48-60 hours or 96 hours respectively (45). After protein expression, cells were harvested and lysed in 10 mM Tris/HCl (pH7.6), 5 mM EDTA, 50 mM NaCl, 30 mM sodium pyrophosphate, 50 mM NaF, 100μ M Na₃VO₄, 1% Triton X-100 and 1 mM phenylmethysulphonyl fluoride (lysis buffer). The extracts were incubated with glutathione-Sepharose beads (Pharmacia) for 2-4 hours at 4° C. Bound proteins were recovered by centrifugation and after repeated washing with lysis buffer, the protein was transferred to storage buffer (50% ethyleneglycol, 5 mM EDTA, 40 mM Tris-HCl pH7.4, 5 mM dithiothriotol, 10 mM benzamidine) at −20° C.

I. Generation of Antisera to PI3K-C2α

An amino-terminal fragment of the PI3K-C2α cDNA (1-1011 bp of SEQ ID NO: 1) was subcloned into pGex-2T vector. This plasmid was used to transform bacteria and the expression of a glutathione S-transferase (GST) fusion protein was induced. This protein was isolated using glutathione-sepharose beads and then eluted upon addition of excess glutathione. The GST fusion protein was used to immunise two rabbits, one of which produced antisera suitable for immunoprecipitation and western blotting PI3K-C2α from cell lysates.

II. Western blotting

Various cell lines were extracted with Laemelli sample buffer and fractionated by SDS-PAGE. The proteins were then transferred onto PVDF membrane which was probed with the anti- PI3K-C2α antisera. After washing, the blots were incubated with goat -anti -rabbit HRP antibody and proteins were visualised by ECL ( Amersham).

A. Subcellular Fractionation of PI3K-C2α

The subcellular distribution of endogenous PI3K-C2α protein was examined by differential centrifugation of cell homogenates. Cells were disrupted either by dounce homogenisation or 10 passages through a 25 gauge needle in Tris/sucrose buffer (0.25M sucrose, 20 mM Tris/HCl pH 7.4, 1 mM sodium orthovanadate, 1 mM PMSF, 10 μg/ml leupeptin, 200 KIU/ml aprotonin, 1 μg/ml antipain, 400 μg/ml benzamidine and 1 μg/ml pepstatin). The homogenate (homog) was centrifuged at 3000 g for 10 minutes to remove nuclei and unbroken cells. The supernatant (s/n) was spun at 18000 g to obtain the plasma membrane fraction (pm) and the resultant supernatant centrifuged for an additional 30 minutes at 350000 g. The pellet obtained was termed the low density microsomal fraction (LDM) and the supernatant cytosol (cyt). All manipulations were performed at 4° C. Fractions were extracted in Laemelli sample buffer, analyzed by SDS-PAGE and visualized by western blotting.

Monkey Kidney Cos cells were transiently transfected with a 5′ and 3′ glu tagged PI3K-C2α construct ligated in a pMT-SM vector. These cells were used 48 hours following electroporation. For fractionation studies, proteins were visualized by a anti- glu tag monoclonal antibody following western blotting. These cells were also used to examine the localization of the PI3K-C2α protein by confocal microscopy. For this purpose, electroporated cells were plated onto glass cover slips. After 48 hours, the cells were fixed, permeabilized and incubated with anti-glu tag monoclonal antibody for two hours. Cells were then washed and incubated with anti-mouse FITC coupled antibody. The staining pattern was then examined using a Zeiss confocal microscope.

Assay of PI 3-kinase Activity

Typically, PI 3-kinase assays were performed in a total volume of 50 μl containing 20 mM Hepes pH7.4. 100 mM NaCl, 0.1 mM EGTA, 0.1% β-mercaptoethanol, and 200 μM phosphoinositide. After preincubating sonicated lipid with sample for 10 minutes, the reactions were initiated upon the addition of 2.5 mM MgCl₂, 100 mM ATP (0.2 μCiγ[³² P] ATP). Assays were incubated at 30° C. for 20 minutes and terminated with acidified chloroform:methanol. The extracted lipid products were analysed by thin layer chromatography using Silica gel 60 plates and chloroform:methanol:4M ammonium hydroxide (45:35:10) for assays examining phosphorylation of Ptdlns. When separations of PtdIns 3P, PtdIns (3,4) P₂ and PItdIns (3,4,5) P₃ was required, propan-1-ol: 2M acetic acid (65:35) was used. Phosphorylated lipids were visualised by either autoradiography or by phosphoimage analysis (Molecular Dynamics). Assays were linear with respect to time and enzyme addition.

Characterization of PI 3K-C2α Reaction Products by HPLC

Phosphorylation of PI was performed using recombinant protein as described above. The reaction products were extracted in acidified chloroform :methanol and deacylated with methylamine (46). HPLC analysis of glycerophosphoinositols was performed using a Partisphere SAX column (Whatman International) and eluted using a linear gradient of 1M (NH₄)₂ HPO₄ (pH3.8) against water at 1 ml min⁻¹. Radioactive peaks were detected using an in-line detector (Reeve Analytical).

Results Cloning of PI3K-C2α

Degenerate primers and mRNA from the human cell line U937 were used to produce a number of partial cDNAs by RT-PCR. One product of these reactions was found to be a novel cDNA which was then used to screen a λ Zap cDNA library made from U937 cells. Twenty seven positive clones selected from 3×10⁶ plaques were obtained following tertiary screening. A 6.1 kb clone was sequenced and found to contain an open reading frame of 4618 nucleotides which could encode a putative catalytic domain. Since no definitive 5′ Kozak consensus sequence was found in this clone (24), RACE PCR was performed using cDNA from U937 cells. A 643 nucleotide extension to the original cDNA was generated. This cDNA fragment contained an ATG sequence which was in frame with the longest open reading frame of the parent clone and had an upstream stop codon. The RACE product was then ligated onto the parent clone to produce a composite cDNA of 5.1 kb which could encode a protein of 1686 amino acid residues that would have a calculated molecular mass of 190 kDa (FIG. 1).

The protein which was termed PI3K-C2α, has an amino acid sequence which when optimally aligned, displays an overall sequence identity of 32.5% with the sequences of drosophila PI3K_(—)68D and cpk (22,23) and 90.8% and 90.2% with those of the murine proteins mp170 (SEQ ID NO:7) (16) and mcpk (SEQ ID NO:8) (22), respectively (FIG. 2a). Two regions of amino acid sequence, HRI and HRII, define areas which have the greatest similarity to the sequences of other PI 3-kinases. The putative catalytic domain (HRl) is defined by residues 1137-1398 of the PI3K-C2α or α protein. Within this region, the amino acid sequence of PI3K-C2α is 62% identical to that of PI3K_(—)68D and cpk, 97% to mp170 and 99.6% to mcpk (FIG. 2c). When the HRl domain of PI3K-C2α is compared to that of p110α and PtdIns 3-kinase, sequence identity falls to only 45% and 35% respectively. Amino acid residues 871-1020 of PI3K-C2α define a region termed the PIK domain (HR2) which is also present in other lipid kinases as well as TOR2 (25). The function of this domain is currently unknown.

The amino acid sequence of the carboxy terminal region of PI3K-C2α (residues 1549-1686) shows strong sequence homology to a domain originally identified as a Ca²⁺ and phospholipid-binding module in protein kinase C, which is termed the C2 domain (13). In this region, the PI3K-C2α amino acid sequence is 96% identical to that of the C2 domains identified in cpk-m and p170, 39% identical to the C2 domain of PI3K_(—)68D and cpk and 32% and 24% identical to the C2A and C2B domains of synaptotagmin respectively (FIG. 2c). Within the amino terminal region, large insertions are necessary in the PI3K-C2α amino acid sequence to allow optimal alignment with that of Drosophila PI3K_(—)68D and cpk. It is perhaps significant that the type II polyproline motif PPLPPR identified in PI3K_(—)68D and cpk (residues 456-462) is absent in PI3K-C2α. Within this region however, numerous proline residues are evident. The amino terminus of PI3K-C2α lacks any clearly delineated regulatory domain.

Interestingly, alignment of the two murine amino acid sequences with that of PI3K-C2α shows that the p170 protein (SEQ ID NO:7) lacks the first 176 amino residues of the longer murine mcpk sequence (SEQ ID NO:8). The mcpk protein has a 28 amino acid residues deletion in this region (residues 275-301 of the PI3K-C2α sequence) which is absent in both mp170 and PI3K-C2α(FIG. 2b). The significance of these sequence differences requires further investigation.

Tissue/Cell Expression of PI3K-C2α

Northern blot analysis carried out using poly A+RNA isolated from human tissue revealed that the cDNA encoding human PI3K-C2α hybridised to a 8 kb species in the RNA from a wide variety of tissues (FIG. 3). Highest levels of expression were found in the heart, placenta and ovary. The mRNA was only undetectable in the kidney. The size of the mRNA is consistent with the length of the full length cDNA. In testes, an additional smaller transcript of approximately 6.5 kb was also present and could have arisen from differential splicing.

Western blots of cell lysates from a variety of cell-lines using the anti-PI3K-C2α antisera confirms the presence of the 190 kDa polypeptide encoding PI3K-C2 in all cell-lines tested with the exception of the smooth muscle cell-line Rasmc, FIG. 7.

Characterisation of Lipid Substrate Specificity

To allow biochemical analysis of PI3K-C2α, a recombinant baculovirus was produced. The construct generated encoded a fusion protein which comprised the amino acid sequence of GST linked to residues 148-1686 of the PI3K-C2α protein. Sf9 cells were infected with the virus and after 2 days, the expressed protein was purified using glutathione-sepharose beads. The enzymatic activity and substrate specificity were investigated and compared to that of GST-p110α expressed in the same system. In the presence of Mg²⁺ p110α was able to utilise PtdIns, PtdIns(4)P₂ and PtdIns(4,5)P₂ as substrates to produce PtdIns3P, PtdIns(3,4) P₂ and PtdIns(3,4,5)P₃ respectively (FIG. 4a). In contrast, PI3K-C2α was able to phosphorylate PtdIns and PtdIns(4)P but could not use PtdIns(4,5)P₂. Like p110, the lipid kinase activity of PI3K-C2α displayed a cation dependence for Mg²⁺ and was found to be inactive in the presence of Mn²⁺. This ion dependence of the enzyme activity of PI3K-C2α contrasts with that of the human Ptd Ins 3-kinase which shows a preference the Mn²⁺ (14).

To confirm that PI3K-C2α, could phosphorylate PtdIns at the D-3 position on the inositol ring, anion exchange HPLC was performed upon the deacylated reaction products. A single peak of radioactive glycerolphosphate was obtained which co-eluted with the deacylated PtdIns 3-P produced by the action of recombinant p110α upon Ptd Ins (FIG. 4b). No additional products were observed confirming that PI3K-C2 specifically phosphorylates phosphatidylinositides at the D-3 position of their inositol ring.

Sensitivity to Inhibitors of PI 3-kinase Activity

The compounds Wortmannin and LY294002 (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one) have been used extensively to assess the involvement of PI 3-kinase in many different physiological processes. To date, all cloned human PI 3-kinases have been found to be sensitive to Wortmannin action at nanomolar concentrations. The effect of these inhibitors on the lipid kinase activity of PI3K-C2α was examined, using PtdIns as a substrate, in the presence of increasing concentrations of each inhibitor. In agreement with previous studies, p110 α activity was found to be sensitive to Wortmannin at low nM concentrations (lC₅₀=3 nM) with maximal attenuation of activity obtained at 100 nM (FIG. 5A). In contrast, the observed IC₅₀ using PI3K-C2α was 200 nM with maximal inhibition only obtained using Wortmannin at 10 μM. For LY294002, the IC₅₀ using p110α was 1.5 mM and maximal inhibition was obtained at 300 μM (FIG. 5B). Again PI3K-C2α was less sensitive than p110α to the action of the inhibitor (IC₅₀=20 μM). The lipid kinase activity of PI3K-C2 α was maximally attenuated using 2 mM LY294002.

III. Subcellular Localization of PI3K-C2α in Monkey Kidney Cos Cells

To determine the subcellular location of PI3K-C2α, Monkey Kidney Cos cells were subjected to physical disruption followed by fractionation by differential centrifugation, FIG. 6. FIG. 7 shows a western blot of various subcellular fractions prepared as described in materials and methods. A significant enrichment of PI3K-C2α polypeptide is shown in the LDM fraction when using the anti-amino terminus antisera, see FIG. 8A. This enrichment is also reflected, although to a lesser extent, when using cell fractions prepared from electroporated Monkey Kidney Cos cells expressing glu tagged PI3K-C2α, see FIG. 8B. The nature of the affinity of PI3K-C2α for microsomal membranes is currently unkown.

Discussion

In the present study the cloning and biochemical characterization of PI3K-C2α, a novel human PI 3-kinase of 190 kDa is described. Based on the presence of a C-terminal C2 domain and the phospholipid substrate specificity of PI3K-C2α, this enzyme like Drosophila PI3K_(—)68D (23), cpk (22) and the two murine PI 3-kinases mp170 (16) and mcpk (22) can be defined as a class II PI 3-kinase (26) (FIG. 2).

The amino acid sequence of PI3K-C2α is 90.8% and 90.2% identical to that of two recently cloned murine PI 3-kinases mp170 (16) and mcpk respectively (22). This level of sequence identity suggests that PI3K-C2α is the human homologue of a single mouse P13-kinase termed mp170 (27) or mcpk (28) which is encoded by the same gene. Despite the overall sequence identity between human PI3K-C2α and murine mp170 and mcpk, alignment of their N-termini shows that mp170 lacks the first 175 amino acid residues of both PI3K-C2α and cpk-m (FIG. 2). In this region mcpk lacks 28 amino acid residues which are present in both PI3K-C2α and mp170. Analysis of the two murine cDNAs suggests that these differences in the proteins may be the result of differential splicing of a single gene.

The putative catalytic domain (HRI) and the PIK domain (HR II) of PI3K-C2α are related to similar regions found in all Class I, II and III P13-kinases. However, PI3K-C2α appears to lack any clear consensus sequences which would delineate other functional domains. For example, PI3K-C2α lacks the amino terminal motif present in p110α, p110β and p110δ which mediates binding to p85 adaptors (29). Furthermore, PI3K-C2α does not contain the type II polyproline motif previously identified in P13K_(—b 68)D and cpk, although it does contain a large number of proline residues which might allow an interaction with SH3 domain-containing proteins through a non consensus binding motif (30).

The class II P13-kinases are in part characterized by their C-terminal C2 domain. C2 domains were originally identified in protein kinase C where they were shown to mediate Ca²⁺-dependent phospholipid binding (13). Subsequently, C2 domains, often occurring in tandem, have also been defined in a variety of other proteins including synaptotagmin, rabphillin 2A and cytosolic phospholipase A2 (31). In these proteins the C2 domain may confer similar biochemical properties. Functional heterogeneity has however, been observed amongst isolated C2 domains despite their high degree of sequence similarity. In addition to phospholipid binding, Ca2+ stimulates an association between synaptotagmin and syntaxin through the first C2A domain (32). In contrast, the second C2B domain is inactive in these assays suggesting that its function is not regulated by intracellular Ca²⁺ levels. Indeed, the second C2 domain of synaptotagmin was shown to display a Ca²⁺ independent binding to clathrin AP2 and polyinositol phosphates (33, 34). Recent studies have since demonstrated however, that the second C2 domain of synaptotagmin can also respond in a Ca²⁺ triggered manner mediating a dimerization event (35, 36). Studies using a GST-fusion protein of the PI3 K_(—)68D C2 domain demonstrated that, like the second C2 domain of synaptotagmin, it was only able to bind acidic phospholipids in a cation independent manner (23). It will be of interest to determine if the biochemical properties of the PI3K-C2α domain are more similar to the first or the second synaptotagmin C2 domain.

The biochemical analysis described here has shown that in vitro PI3K-C2α can phosphorylate both Ptd Ins and Ptd Ins (4)P in the D3 position of the inositol ring (FIG. 4). These findings are consistent with the enzyme having a lipid specificity similar to that of P13K₁₃ 68D and cpk (23), (22). The substrate specificity of the mouse homologue has only been reported by Virbasius et al who concluded that Ptd Ins was the major lipid substrate of mp170 in vitro since they found that PtdIns(4)P was only very poorly phosphorylated by their enzyme (16). These results are somewhat surprising considering the degree of sequence identity between the catalytic domains of PI3K-C2α and mpl70 (FIG. 2). One explanation for this difference could be that Virbasius et al used a crude brain lipid extract substrate in contrast to the purified lipid preparations we have used in the analysis of substrate specificity (16). Although it could be argued that use of the crude extract may be more physiological, it does not allow for an accurate quantitative comparison of the lipids present in the two preparations. Under these circumstances, a comparative assessment of the efficiency with which an enzyme could utilize a particular lipid substrate cannot be made accurately. Furthermore, a crude lipid preparation may contain factors which influence the lipid kinase activity of the recombinant protein.

The involvement of PI3-kinases in various processes of cell physiology has relied more and more on the use of the inhibitors Wortmannin and LY294002. Although initially described as an inhibitor of myosin light chain kinase at micromolar concentrations (37), Wortmannin was later shown to potently inhibit PDGF receptor-associated PI 3-kinase activity at 100 fold lower concentrations (2-5 nanoinolar). Used in the low nanomolar range, Wortmannin was shown to block the generation of 3-phosphorylated lipids both in vitro and in whole cells such as murine fibroblasts (38). On the basis of such findings, Wortmannin began to be used extensively as a specific inhibitor of PI 3-kinase activity thereby implicating the formation of 3-phosphorylated lipids in a number of physiological responses. When the effect of Wortmannin on the lipid kinase activity of recombinant PI3K-C2α was examined we found that the concentrations of Wortmannin required to inhibit PI3K-C2α activity were more similar to those previously used to inhibit myosin light chain kinase (IC₅₀=170-200 nM) (37, 39). Similarly, data presented using the mp170 enzyme suggests that it is also less sensitive to Wortmannin treatment than p110α (16). LY294002 is a structurally unrelated inhibitor of PI 3-kinase activity and PI3K-C2α is also less sensitive than p110α to this inhibitor. The difference in sensitivity between PI3K-C2α and p110α is however, only approximately one order of magnitude. The relevance of this differential sensitivity is unclear. Studies using p110α have shown that Wortmannin inactivates kinase activity by a covalent modification of Lys-802, a residue involved in the phosphate transfer reaction (40). This residue is present in all PI 3-kinase family members including PI3K-C2α. Further molecular modelling and structural studies may elucidate the nature of this Wortmannin resistance. These results with PI3K-C2α contrast not only with those derived from studies of Class I and III human PI 3-kinases but also to those obtained with the Drosophila PI3K_(—)68D which displays a sensitivity to Wortmannin which is similar to that of p110α (23). The concentrations of both Wortmannin and LY294002 required to abolish PI3K-C2α activity are therefore likely to have precluded its inhibition in many experiments where PI 3-kinase activity has been studied. These findings may thus require many investigators to re-evaluate a possible involvement of PI3-kinase action in their physiological studies. Questions relating to the precise role PI3K-C2α plays in cells will consequently be hampered by the absence of suitable inhibitors of its lipid kinase activity.

The foregoing description and examples have been set forth merely to illustrate the invention and are not intended to be limiting. Since modifications of the described embodiments incorporating the spirit and substance of the invention may occur to persons skilled in the art, the invention should be construed broadly to include all variations falling within the scope of the appended claims and equivalents thereof.

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                   #             SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 11 <210> SEQ ID NO 1 <211> LENGTH: 5061 <212> TYPE: DNA <213> ORGANISM: Homo sapiens <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(5058) <223> OTHER INFORMATION: <400> SEQUENCE: 1 atg gct cag ata ttt agc aac agc gga ttt aa #a gaa tgt cca ttt tca       48 Met Ala Gln Ile Phe Ser Asn Ser Gly Phe Ly #s Glu Cys Pro Phe Ser 1               5    #                10   #                15 cat ccg gaa cca aca aga gca aaa gat gtg ga #c aaa gaa gaa gca tta       96 His Pro Glu Pro Thr Arg Ala Lys Asp Val As #p Lys Glu Glu Ala Leu             20       #            25       #            30 cag atg gaa gca gag gct tta gca aaa ctg ca #a aag gat aga caa gtg      144 Gln Met Glu Ala Glu Ala Leu Ala Lys Leu Gl #n Lys Asp Arg Gln Val         35           #        40           #        45 act gac aat cag aga ggc ttt gag ttg tca ag #c agc acc aga aaa aaa      192 Thr Asp Asn Gln Arg Gly Phe Glu Leu Ser Se #r Ser Thr Arg Lys Lys     50               #    55               #    60 gca cag gtt tat aac aag cag gat tat gat ct #c atg gtg ttt cct gaa      240 Ala Gln Val Tyr Asn Lys Gln Asp Tyr Asp Le #u Met Val Phe Pro Glu 65                   #70                   #75                   #80 tca gat tcc caa aaa aga gca tta gat att ga #t gta gaa aag ctc acc      288 Ser Asp Ser Gln Lys Arg Ala Leu Asp Ile As #p Val Glu Lys Leu Thr                 85   #                90   #                95 caa gct gaa ctt gag aaa cta ttg ctg gat ga #c agt ttc gag act aaa      336 Gln Ala Glu Leu Glu Lys Leu Leu Leu Asp As #p Ser Phe Glu Thr Lys             100       #           105       #           110 aaa aca cct gta tta cca gtt act cct att ct #g agc cct tcc ttt tca      384 Lys Thr Pro Val Leu Pro Val Thr Pro Ile Le #u Ser Pro Ser Phe Ser         115           #       120           #       125 gca cag ctc tat ttt aga cct act att cag ag #a gga cag tgg cca cct      432 Ala Gln Leu Tyr Phe Arg Pro Thr Ile Gln Ar #g Gly Gln Trp Pro Pro     130               #   135               #   140 gga tta cct ggg cct tcc act tat gct tta cc #t tct att tat cct tct      480 Gly Leu Pro Gly Pro Ser Thr Tyr Ala Leu Pr #o Ser Ile Tyr Pro Ser 145                 1 #50                 1 #55                 1 #60 act tac agt aaa cag gct gca ttc caa aat gg #c ttc aat cca aga atg      528 Thr Tyr Ser Lys Gln Ala Ala Phe Gln Asn Gl #y Phe Asn Pro Arg Met                 165   #               170   #               175 ccc act ttt cca tct aca gaa cct ata tat tt #a agt ctt ccg gga caa      576 Pro Thr Phe Pro Ser Thr Glu Pro Ile Tyr Le #u Ser Leu Pro Gly Gln             180       #           185       #           190 tct cca tat ttc tca tat cct ttg aca cct gc #c aca ccc ttt cat cca      624 Ser Pro Tyr Phe Ser Tyr Pro Leu Thr Pro Al #a Thr Pro Phe His Pro         195           #       200           #       205 caa gga agc tta cct atc tat cgt cca gta gt #c agt act gac atg gca      672 Gln Gly Ser Leu Pro Ile Tyr Arg Pro Val Va #l Ser Thr Asp Met Ala     210               #   215               #   220 aaa cta ttt gac aaa ata gct agt aca tca ga #a ttt tta aaa aat ggg      720 Lys Leu Phe Asp Lys Ile Ala Ser Thr Ser Gl #u Phe Leu Lys Asn Gly 225                 2 #30                 2 #35                 2 #40 aaa gca agg act gat ttg gag ata aca gat tc #a aaa gtc agc aat cta      768 Lys Ala Arg Thr Asp Leu Glu Ile Thr Asp Se #r Lys Val Ser Asn Leu                 245   #               250   #               255 cag gta tct cca aag tct gag gat atc agt aa #a ttt gac tgg tta gac      816 Gln Val Ser Pro Lys Ser Glu Asp Ile Ser Ly #s Phe Asp Trp Leu Asp             260       #           265       #           270 ttg gat cct cta agt aag cct aag gtg gat aa #t gtg gag gta tta gac      864 Leu Asp Pro Leu Ser Lys Pro Lys Val Asp As #n Val Glu Val Leu Asp         275           #       280           #       285 cat gag gaa gag aaa aat gtt tca agt ttg ct #a gca aag gat cct tgg      912 His Glu Glu Glu Lys Asn Val Ser Ser Leu Le #u Ala Lys Asp Pro Trp     290               #   295               #   300 gat gct gtt ctt ctt gaa gag aga tcg aca gc #a aat tgt cat ctt gaa      960 Asp Ala Val Leu Leu Glu Glu Arg Ser Thr Al #a Asn Cys His Leu Glu 305                 3 #10                 3 #15                 3 #20 aga aag gtg aat gga aaa tcc ctt tct gtg gc #a act gtt aca aga agc     1008 Arg Lys Val Asn Gly Lys Ser Leu Ser Val Al #a Thr Val Thr Arg Ser                 325   #               330   #               335 cag tct tta aat att cga aca act cag ctt gc #a aaa gcc cag ggc cat     1056 Gln Ser Leu Asn Ile Arg Thr Thr Gln Leu Al #a Lys Ala Gln Gly His             340       #           345       #           350 ata tct cag aaa gac cca aat ggg acc agt ag #t ttg cca act gga agt     1104 Ile Ser Gln Lys Asp Pro Asn Gly Thr Ser Se #r Leu Pro Thr Gly Ser         355           #       360           #       365 tct ctt ctt caa gaa gtt gaa gta cag aat ga #g gag atg gca gct ttt     1152 Ser Leu Leu Gln Glu Val Glu Val Gln Asn Gl #u Glu Met Ala Ala Phe     370               #   375               #   380 tgt cga tcc att aca aaa ttg aag acc aaa tt #t cca tat acc aat cac     1200 Cys Arg Ser Ile Thr Lys Leu Lys Thr Lys Ph #e Pro Tyr Thr Asn His 385                 3 #90                 3 #95                 4 #00 cgc aca aac cca ggc tat ttg tta agt cca gt #c aca gcg caa aga aac     1248 Arg Thr Asn Pro Gly Tyr Leu Leu Ser Pro Va #l Thr Ala Gln Arg Asn                 405   #               410   #               415 ata tgc gga gaa aat gct agt gtg aag gtc tc #c att gac att gaa gga     1296 Ile Cys Gly Glu Asn Ala Ser Val Lys Val Se #r Ile Asp Ile Glu Gly             420       #           425       #           430 ttt cag cta cca gtt act ttt acg tgt gat gt #g agt tct act gta gaa     1344 Phe Gln Leu Pro Val Thr Phe Thr Cys Asp Va #l Ser Ser Thr Val Glu         435           #       440           #       445 atc att ata atg caa gcc ctt tgc tgg gta ca #t gat gac ttg aat caa     1392 Ile Ile Ile Met Gln Ala Leu Cys Trp Val Hi #s Asp Asp Leu Asn Gln     450               #   455               #   460 gta gat gtt ggc agc tat gtt cta aaa gtt tg #t ggt caa gag gaa gtg     1440 Val Asp Val Gly Ser Tyr Val Leu Lys Val Cy #s Gly Gln Glu Glu Val 465                 4 #70                 4 #75                 4 #80 ctg cag aat aat cat tgc ctt gga agt cat ga #g cat att caa aac tgt     1488 Leu Gln Asn Asn His Cys Leu Gly Ser His Gl #u His Ile Gln Asn Cys                 485   #               490   #               495 cga aaa tgg gac aca gaa att aga cta caa ct #c ttg acc ttc agt gca     1536 Arg Lys Trp Asp Thr Glu Ile Arg Leu Gln Le #u Leu Thr Phe Ser Ala             500       #           505       #           510 atg tgt caa aat ctg gcc cga aca gca gaa ga #t gat gaa aca ccc gtg     1584 Met Cys Gln Asn Leu Ala Arg Thr Ala Glu As #p Asp Glu Thr Pro Val         515           #       520           #       525 gat tta aac aaa cac ctg tat caa ata gaa aa #a cct tgc aaa gaa gcc     1632 Asp Leu Asn Lys His Leu Tyr Gln Ile Glu Ly #s Pro Cys Lys Glu Ala     530               #   535               #   540 atg acg aga cac cct gtt gaa gaa ctc tta ga #t tct tat cac aac caa     1680 Met Thr Arg His Pro Val Glu Glu Leu Leu As #p Ser Tyr His Asn Gln 545                 5 #50                 5 #55                 5 #60 gta gaa ctg gct ctt caa att gaa aac caa ca #c cga gca gta gat caa     1728 Val Glu Leu Ala Leu Gln Ile Glu Asn Gln Hi #s Arg Ala Val Asp Gln                 565   #               570   #               575 gta att aaa gct gta aga aaa atc tgt agt gc #t tta gat ggt gtc gag     1776 Val Ile Lys Ala Val Arg Lys Ile Cys Ser Al #a Leu Asp Gly Val Glu             580       #           585       #           590 act ctt gcc att aca gaa tca gta aag aag ct #a aag aga gca gtt aat     1824 Thr Leu Ala Ile Thr Glu Ser Val Lys Lys Le #u Lys Arg Ala Val Asn         595           #       600           #       605 ctt cca agg agt aaa act gct gat gtg act tc #t ttg ttt gga gga gaa     1872 Leu Pro Arg Ser Lys Thr Ala Asp Val Thr Se #r Leu Phe Gly Gly Glu     610               #   615               #   620 gac act agc agg agt tca act agg ggc tca ct #t aat cct gaa aat cct     1920 Asp Thr Ser Arg Ser Ser Thr Arg Gly Ser Le #u Asn Pro Glu Asn Pro 625                 6 #30                 6 #35                 6 #40 gtt caa gta agc ata aac caa tta act gca gc #a att tat gat ctt ctc     1968 Val Gln Val Ser Ile Asn Gln Leu Thr Ala Al #a Ile Tyr Asp Leu Leu                 645   #               650   #               655 aga ctc cat gca aat tct ggt agg agt cct ac #a gac tgt gcc caa agt     2016 Arg Leu His Ala Asn Ser Gly Arg Ser Pro Th #r Asp Cys Ala Gln Ser             660       #           665       #           670 agc aag agt gtc aag gaa gca tgg act aca ac #a gag cag ctc cag ttt     2064 Ser Lys Ser Val Lys Glu Ala Trp Thr Thr Th #r Glu Gln Leu Gln Phe         675           #       680           #       685 act att ttt gct gct cat gga att tca agt aa #t tgg gta tca aat tat     2112 Thr Ile Phe Ala Ala His Gly Ile Ser Ser As #n Trp Val Ser Asn Tyr     690               #   695               #   700 gaa aaa tac tac ttg ata tgt tca ctg tct ca #c aat gga aag gat ctt     2160 Glu Lys Tyr Tyr Leu Ile Cys Ser Leu Ser Hi #s Asn Gly Lys Asp Leu 705                 7 #10                 7 #15                 7 #20 ttt aaa cct att caa tca aag aag gtt ggc ac #t tac aag aat ttc ttc     2208 Phe Lys Pro Ile Gln Ser Lys Lys Val Gly Th #r Tyr Lys Asn Phe Phe                 725   #               730   #               735 tat ctt att aaa tgg gat gaa cta atc att tt #t cct atc cag ata tca     2256 Tyr Leu Ile Lys Trp Asp Glu Leu Ile Ile Ph #e Pro Ile Gln Ile Ser             740       #           745       #           750 caa ttg cca tta gaa tca gtt ctt cac ctt ac #t ctt ttt gga att tta     2304 Gln Leu Pro Leu Glu Ser Val Leu His Leu Th #r Leu Phe Gly Ile Leu         755           #       760           #       765 aat cag agc agt gga agt tcc cct gat tct aa #t aag cag aga aag gga     2352 Asn Gln Ser Ser Gly Ser Ser Pro Asp Ser As #n Lys Gln Arg Lys Gly     770               #   775               #   780 cca gaa gct ttg ggc aaa gtt tct tta cct ct #t tgt gac ttt aga cgg     2400 Pro Glu Ala Leu Gly Lys Val Ser Leu Pro Le #u Cys Asp Phe Arg Arg 785                 7 #90                 7 #95                 8 #00 ttt tta aca tgt gga act aaa ctt cta tat ct #t tgg act tca tca cat     2448 Phe Leu Thr Cys Gly Thr Lys Leu Leu Tyr Le #u Trp Thr Ser Ser His                 805   #               810   #               815 aca aat tct gtt cct gga aca gtt acc aaa aa #a gga tat gtc atg gaa     2496 Thr Asn Ser Val Pro Gly Thr Val Thr Lys Ly #s Gly Tyr Val Met Glu             820       #           825       #           830 aga ata gtg cta cag gtt gat ttt cct tct cc #t gca ttt gat att att     2544 Arg Ile Val Leu Gln Val Asp Phe Pro Ser Pr #o Ala Phe Asp Ile Ile         835           #       840           #       845 tat aca act cct caa gtt gac aga agc att at #a cag caa cat aac tta     2592 Tyr Thr Thr Pro Gln Val Asp Arg Ser Ile Il #e Gln Gln His Asn Leu     850               #   855               #   860 gaa aca cta gag aat gat ata aaa ggg aaa ct #t ctt gat att ctt cat     2640 Glu Thr Leu Glu Asn Asp Ile Lys Gly Lys Le #u Leu Asp Ile Leu His 865                 8 #70                 8 #75                 8 #80 aaa gac tca tca ctt gga ctt tct aaa gaa ga #t aaa gct ttt tta tgg     2688 Lys Asp Ser Ser Leu Gly Leu Ser Lys Glu As #p Lys Ala Phe Leu Trp                 885   #               890   #               895 gag aaa cgt tat tat tgc ttc aaa cac cca aa #t tgt ctt cct aaa ata     2736 Glu Lys Arg Tyr Tyr Cys Phe Lys His Pro As #n Cys Leu Pro Lys Ile             900       #           905       #           910 tta gca agc gcc cca aac tgg aaa tgg ggt aa #t ctt gcc aaa act tac     2784 Leu Ala Ser Ala Pro Asn Trp Lys Trp Gly As #n Leu Ala Lys Thr Tyr         915           #       920           #       925 tca ttg ctt cac cag tgg cct gca ttg tac cc #a cta att gca ttg gaa     2832 Ser Leu Leu His Gln Trp Pro Ala Leu Tyr Pr #o Leu Ile Ala Leu Glu     930               #   935               #   940 ctt ctt gat tca aaa ttt gct gat cag gaa gt #a aga tcc cta gct gtg     2880 Leu Leu Asp Ser Lys Phe Ala Asp Gln Glu Va #l Arg Ser Leu Ala Val 945                 9 #50                 9 #55                 9 #60 acc tgg att gag gcc att agt gat gat gag ct #a aca gat ctt ctt cca     2928 Thr Trp Ile Glu Ala Ile Ser Asp Asp Glu Le #u Thr Asp Leu Leu Pro                 965   #               970   #               975 cag ttt gta caa gct ttg aaa tat gaa att ta #c ttg aat agt tca tta     2976 Gln Phe Val Gln Ala Leu Lys Tyr Glu Ile Ty #r Leu Asn Ser Ser Leu             980       #           985       #           990 gtg caa ttc ctt ttg tcc agg gca  ttg gga  #aat atc cag  ata gca cac   3024 Val Gln Phe Leu Leu Ser Arg Ala  Leu Gly  #Asn Ile Gln  Ile Ala His         995           #       1000           #       1005 aat tta  tat tgg ctt ctc aaa  gat gcc c #tg cat gat  gta cag ttt      3069 Asn Leu  Tyr Trp Leu Leu Lys  Asp Ala L #eu His Asp  Val Gln Phe     1010              #    1015              #    1020 agt acc  cga tac gaa cat gtt  ttg ggt g #ct ctc ctg  tca gta gga      3114 Ser Thr  Arg Tyr Glu His Val  Leu Gly A #la Leu Leu  Ser Val Gly     1025              #    1030              #    1035 gga aaa  cga ctt aga gaa gaa  ctt cta a #aa cag acg  aaa ctt gta      3159 Gly Lys  Arg Leu Arg Glu Glu  Leu Leu L #ys Gln Thr  Lys Leu Val     1040              #    1045              #    1050 cag ctt  tta gga gga gta gca  gaa aaa g #ta agg cag  gct agt gga      3204 Gln Leu  Leu Gly Gly Val Ala  Glu Lys V #al Arg Gln  Ala Ser Gly     1055              #    1060              #    1065 tca gcc  aga cag gtt gtt ctc  caa aga a #gt atg gaa  cga gta cag      3249 Ser Ala  Arg Gln Val Val Leu  Gln Arg S #er Met Glu  Arg Val Gln     1070              #    1075              #    1080 tcc ttt  ttt cag aaa aat aaa  tgc cgt c #tc cct ctc  aag cca agt      3294 Ser Phe  Phe Gln Lys Asn Lys  Cys Arg L #eu Pro Leu  Lys Pro Ser     1085              #    1090              #    1095 cta gtg  gca aaa gaa tta aat  att aag t #cg tgt tcc  ttc ttc agt      3339 Leu Val  Ala Lys Glu Leu Asn  Ile Lys S #er Cys Ser  Phe Phe Ser     1100              #    1105              #    1110 tct aat  gct gtc ccc cta aaa  gtc aca a #tg gtg aat  gct gac cct      3384 Ser Asn  Ala Val Pro Leu Lys  Val Thr M #et Val Asn  Ala Asp Pro     1115              #    1120              #    1125 ctg gga  gaa gaa att aat gtc  atg ttt a #ag gtt ggt  gaa gat ctt      3429 Leu Gly  Glu Glu Ile Asn Val  Met Phe L #ys Val Gly  Glu Asp Leu     1130              #    1135              #    1140 cgg caa  gat atg tta gct tta  cag atg a #ta aag att  atg gat aag      3474 Arg Gln  Asp Met Leu Ala Leu  Gln Met I #le Lys Ile  Met Asp Lys     1145              #    1150              #    1155 atc tgg  ctt aaa gaa gga cta  gat ctg a #gg atg gta  att ttc aaa      3519 Ile Trp  Leu Lys Glu Gly Leu  Asp Leu A #rg Met Val  Ile Phe Lys     1160              #    1165              #    1170 tgt ctc  tca act ggc aga gat  cga ggc a #tg gtg gag  ctg gtt cct      3564 Cys Leu  Ser Thr Gly Arg Asp  Arg Gly M #et Val Glu  Leu Val Pro     1175              #    1180              #    1185 gct tcc  gat acc ctc agg aaa  atc caa g #tg gaa tat  ggt gtg aca      3609 Ala Ser  Asp Thr Leu Arg Lys  Ile Gln V #al Glu Tyr  Gly Val Thr     1190              #    1195              #    1200 gga tcc  ttt aaa gat aaa cca  ctt gca g #ag tgg cta  agg aaa tac      3654 Gly Ser  Phe Lys Asp Lys Pro  Leu Ala G #lu Trp Leu  Arg Lys Tyr     1205              #    1210              #    1215 aat ccc  tct gaa gaa gaa tat  gaa aag g #ct tca gag  aac ttt atc      3699 Asn Pro  Ser Glu Glu Glu Tyr  Glu Lys A #la Ser Glu  Asn Phe Ile     1220              #    1225              #    1230 tat tcc  tgt gct gga tgc tgt  gta gcc a #cc tat gtt  tta ggc atc      3744 Tyr Ser  Cys Ala Gly Cys Cys  Val Ala T #hr Tyr Val  Leu Gly Ile     1235              #    1240              #    1245 tgt gat  cga cac aat gac aat  ata atg c #tt cga agc  acg gga cac      3789 Cys Asp  Arg His Asn Asp Asn  Ile Met L #eu Arg Ser  Thr Gly His     1250              #    1255              #    1260 atg ttt  cac att gac ttt gga  aag ttt t #tg gga cat  gca cag atg      3834 Met Phe  His Ile Asp Phe Gly  Lys Phe L #eu Gly His  Ala Gln Met     1265              #    1270              #    1275 ttt ggc  agc ttc aaa agg gat  cgg gct c #ct ttt gtg  ctg acc tct      3879 Phe Gly  Ser Phe Lys Arg Asp  Arg Ala P #ro Phe Val  Leu Thr Ser     1280              #    1285              #    1290 gat atg  gca tat gtc att aat  ggg ggt g #aa aag ccc  acc att cgt      3924 Asp Met  Ala Tyr Val Ile Asn  Gly Gly G #lu Lys Pro  Thr Ile Arg     1295              #    1300              #    1305 ttt cag  ttg ttt gtg gac ctc  tgc tgt c #ag gcc tac  aac ttg ata      3969 Phe Gln  Leu Phe Val Asp Leu  Cys Cys G #ln Ala Tyr  Asn Leu Ile     1310              #    1315              #    1320 aga aag  cag aca aac ctt ttt  ctt aac c #tc ctt tca  ctg atg att      4014 Arg Lys  Gln Thr Asn Leu Phe  Leu Asn L #eu Leu Ser  Leu Met Ile     1325              #    1330              #    1335 cct tca  ggg tta cca gaa ctt  aca agt a #tt caa gat  ttg aaa tac      4059 Pro Ser  Gly Leu Pro Glu Leu  Thr Ser I #le Gln Asp  Leu Lys Tyr     1340              #    1345              #    1350 gtt aga  gat gca ctt caa ccc  caa act a #ca gac gca  gaa gct aca      4104 Val Arg  Asp Ala Leu Gln Pro  Gln Thr T #hr Asp Ala  Glu Ala Thr     1355              #    1360              #    1365 att ttc  ttt act agg ctt att  gaa tca a #gt ttg gga  agc att gcc      4149 Ile Phe  Phe Thr Arg Leu Ile  Glu Ser S #er Leu Gly  Ser Ile Ala     1370              #    1375              #    1380 aca aag  ttt aac ttc ttc att  cac aac c #tt gct cag  ctt cgt ttt      4194 Thr Lys  Phe Asn Phe Phe Ile  His Asn L #eu Ala Gln  Leu Arg Phe     1385              #    1390              #    1395 tct ggt  ctt cct tct aat gat  gag ccc a #tc ctt tca  ttt tca cct      4239 Ser Gly  Leu Pro Ser Asn Asp  Glu Pro I #le Leu Ser  Phe Ser Pro     1400              #    1405              #    1410 aaa aca  tac tcc ttt aga caa  gat ggt c #ga atc aag  gaa gtc tct      4284 Lys Thr  Tyr Ser Phe Arg Gln  Asp Gly A #rg Ile Lys  Glu Val Ser     1415              #    1420              #    1425 gtt ttt  aca tat cat aag aaa  tac aac c #ca gat aaa  cat tat att      4329 Val Phe  Thr Tyr His Lys Lys  Tyr Asn P #ro Asp Lys  His Tyr Ile     1430              #    1435              #    1440 tat gta  gtc cga att ttg tgg  gaa gga c #ag att gaa  cca tca ttt      4374 Tyr Val  Val Arg Ile Leu Trp  Glu Gly G #ln Ile Glu  Pro Ser Phe     1445              #    1450              #    1455 gtc ttc  cga aca ttt gtc gaa  ttt cag g #aa ctt cac  aat aag ctc      4419 Val Phe  Arg Thr Phe Val Glu  Phe Gln G #lu Leu His  Asn Lys Leu     1460              #    1465              #    1470 agt att  att ttt cca ctt tgg  aag tta c #ca ggc ttt  cct aat agg      4464 Ser Ile  Ile Phe Pro Leu Trp  Lys Leu P #ro Gly Phe  Pro Asn Arg     1475              #    1480              #    1485 atg gtt  cta gga aga aca cac  ata aaa g #at gta gca  gcc aaa agg      4509 Met Val  Leu Gly Arg Thr His  Ile Lys A #sp Val Ala  Ala Lys Arg     1490              #    1495              #    1500 aaa att  gag tta aac agt tac  tta cag a #gt ttg atg  aat gct tca      4554 Lys Ile  Glu Leu Asn Ser Tyr  Leu Gln S #er Leu Met  Asn Ala Ser     1505              #    1510              #    1515 acg gat  gta gca gag tgt gat  ctt gtt t #gt act ttc  ttc cac cct      4599 Thr Asp  Val Ala Glu Cys Asp  Leu Val C #ys Thr Phe  Phe His Pro     1520              #    1525              #    1530 tta ctt  cgt gat gag aaa gct  gaa ggg a #ta gct agg  tct gca gat      4644 Leu Leu  Arg Asp Glu Lys Ala  Glu Gly I #le Ala Arg  Ser Ala Asp     1535              #    1540              #    1545 gca ggt  tcc ttc agt cct act  cca ggc c #aa ata gga  gga gct gtg      4689 Ala Gly  Ser Phe Ser Pro Thr  Pro Gly G #ln Ile Gly  Gly Ala Val     1550              #    1555              #    1560 aaa tta  tcc atc tct tac cga  aat ggt a #ct ctt ttc  atc atg gtg      4734 Lys Leu  Ser Ile Ser Tyr Arg  Asn Gly T #hr Leu Phe  Ile Met Val     1565              #    1570              #    1575 atg cat  atc aaa gat ctt gtt  act gaa g #at gga gct  gac cca aat      4779 Met His  Ile Lys Asp Leu Val  Thr Glu A #sp Gly Ala  Asp Pro Asn     1580              #    1585              #    1590 cca tat  gtc aaa aca tac cta  ctt cca g #at aac cac  aaa aca tcc      4824 Pro Tyr  Val Lys Thr Tyr Leu  Leu Pro A #sp Asn His  Lys Thr Ser     1595              #    1600              #    1605 aaa cgt  aaa acc aaa att tca  cga aaa a #cg agg aat  ccg aca ttc      4869 Lys Arg  Lys Thr Lys Ile Ser  Arg Lys T #hr Arg Asn  Pro Thr Phe     1610              #    1615              #    1620 aat gaa  atg ctt gta tac agt  gga tat a #gc aaa gaa  acc cta aga      4914 Asn Glu  Met Leu Val Tyr Ser  Gly Tyr S #er Lys Glu  Thr Leu Arg     1625              #    1630              #    1635 cag cga  gaa ctt caa cta agt  gta ctc a #gt gca gaa  tct ctg cgg      4959 Gln Arg  Glu Leu Gln Leu Ser  Val Leu S #er Ala Glu  Ser Leu Arg     1640              #    1645              #    1650 gag aat  ttt ttc ttg ggt gga  gta acc c #tg cct ttg  aaa gat ttc      5004 Glu Asn  Phe Phe Leu Gly Gly  Val Thr L #eu Pro Leu  Lys Asp Phe     1655              #    1660              #    1665 aac ttg  agc aaa gag acg gtt  aaa tgg t #at cag ctg  act gcg gca      5049 Asn Leu  Ser Lys Glu Thr Val  Lys Trp T #yr Gln Leu  Thr Ala Ala     1670              #    1675              #    1680 aca tac  ttg taa            #                   #                   #     5061 Thr Tyr  Leu     1685 <210> SEQ ID NO 2 <211> LENGTH: 1686 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <400> SEQUENCE: 2 Met Ala Gln Ile Phe Ser Asn Ser Gly Phe Ly #s Glu Cys Pro Phe Ser 1               5    #                10   #                15 His Pro Glu Pro Thr Arg Ala Lys Asp Val As #p Lys Glu Glu Ala Leu             20       #            25       #            30 Gln Met Glu Ala Glu Ala Leu Ala Lys Leu Gl #n Lys Asp Arg Gln Val         35           #        40           #        45 Thr Asp Asn Gln Arg Gly Phe Glu Leu Ser Se #r Ser Thr Arg Lys Lys     50               #    55               #    60 Ala Gln Val Tyr Asn Lys Gln Asp Tyr Asp Le #u Met Val Phe Pro Glu 65                   #70                   #75                   #80 Ser Asp Ser Gln Lys Arg Ala Leu Asp Ile As #p Val Glu Lys Leu Thr                 85   #                90   #                95 Gln Ala Glu Leu Glu Lys Leu Leu Leu Asp As #p Ser Phe Glu Thr Lys             100       #           105       #           110 Lys Thr Pro Val Leu Pro Val Thr Pro Ile Le #u Ser Pro Ser Phe Ser         115           #       120           #       125 Ala Gln Leu Tyr Phe Arg Pro Thr Ile Gln Ar #g Gly Gln Trp Pro Pro     130               #   135               #   140 Gly Leu Pro Gly Pro Ser Thr Tyr Ala Leu Pr #o Ser Ile Tyr Pro Ser 145                 1 #50                 1 #55                 1 #60 Thr Tyr Ser Lys Gln Ala Ala Phe Gln Asn Gl #y Phe Asn Pro Arg Met                 165   #               170   #               175 Pro Thr Phe Pro Ser Thr Glu Pro Ile Tyr Le #u Ser Leu Pro Gly Gln             180       #           185       #           190 Ser Pro Tyr Phe Ser Tyr Pro Leu Thr Pro Al #a Thr Pro Phe His Pro         195           #       200           #       205 Gln Gly Ser Leu Pro Ile Tyr Arg Pro Val Va #l Ser Thr Asp Met Ala     210               #   215               #   220 Lys Leu Phe Asp Lys Ile Ala Ser Thr Ser Gl #u Phe Leu Lys Asn Gly 225                 2 #30                 2 #35                 2 #40 Lys Ala Arg Thr Asp Leu Glu Ile Thr Asp Se #r Lys Val Ser Asn Leu                 245   #               250   #               255 Gln Val Ser Pro Lys Ser Glu Asp Ile Ser Ly #s Phe Asp Trp Leu Asp             260       #           265       #           270 Leu Asp Pro Leu Ser Lys Pro Lys Val Asp As #n Val Glu Val Leu Asp         275           #       280           #       285 His Glu Glu Glu Lys Asn Val Ser Ser Leu Le #u Ala Lys Asp Pro Trp     290               #   295               #   300 Asp Ala Val Leu Leu Glu Glu Arg Ser Thr Al #a Asn Cys His Leu Glu 305                 3 #10                 3 #15                 3 #20 Arg Lys Val Asn Gly Lys Ser Leu Ser Val Al #a Thr Val Thr Arg Ser                 325   #               330   #               335 Gln Ser Leu Asn Ile Arg Thr Thr Gln Leu Al #a Lys Ala Gln Gly His             340       #           345       #           350 Ile Ser Gln Lys Asp Pro Asn Gly Thr Ser Se #r Leu Pro Thr Gly Ser         355           #       360           #       365 Ser Leu Leu Gln Glu Val Glu Val Gln Asn Gl #u Glu Met Ala Ala Phe     370               #   375               #   380 Cys Arg Ser Ile Thr Lys Leu Lys Thr Lys Ph #e Pro Tyr Thr Asn His 385                 3 #90                 3 #95                 4 #00 Arg Thr Asn Pro Gly Tyr Leu Leu Ser Pro Va #l Thr Ala Gln Arg Asn                 405   #               410   #               415 Ile Cys Gly Glu Asn Ala Ser Val Lys Val Se #r Ile Asp Ile Glu Gly             420       #           425       #           430 Phe Gln Leu Pro Val Thr Phe Thr Cys Asp Va #l Ser Ser Thr Val Glu         435           #       440           #       445 Ile Ile Ile Met Gln Ala Leu Cys Trp Val Hi #s Asp Asp Leu Asn Gln     450               #   455               #   460 Val Asp Val Gly Ser Tyr Val Leu Lys Val Cy #s Gly Gln Glu Glu Val 465                 4 #70                 4 #75                 4 #80 Leu Gln Asn Asn His Cys Leu Gly Ser His Gl #u His Ile Gln Asn Cys                 485   #               490   #               495 Arg Lys Trp Asp Thr Glu Ile Arg Leu Gln Le #u Leu Thr Phe Ser Ala             500       #           505       #           510 Met Cys Gln Asn Leu Ala Arg Thr Ala Glu As #p Asp Glu Thr Pro Val         515           #       520           #       525 Asp Leu Asn Lys His Leu Tyr Gln Ile Glu Ly #s Pro Cys Lys Glu Ala     530               #   535               #   540 Met Thr Arg His Pro Val Glu Glu Leu Leu As #p Ser Tyr His Asn Gln 545                 5 #50                 5 #55                 5 #60 Val Glu Leu Ala Leu Gln Ile Glu Asn Gln Hi #s Arg Ala Val Asp Gln                 565   #               570   #               575 Val Ile Lys Ala Val Arg Lys Ile Cys Ser Al #a Leu Asp Gly Val Glu             580       #           585       #           590 Thr Leu Ala Ile Thr Glu Ser Val Lys Lys Le #u Lys Arg Ala Val Asn         595           #       600           #       605 Leu Pro Arg Ser Lys Thr Ala Asp Val Thr Se #r Leu Phe Gly Gly Glu     610               #   615               #   620 Asp Thr Ser Arg Ser Ser Thr Arg Gly Ser Le #u Asn Pro Glu Asn Pro 625                 6 #30                 6 #35                 6 #40 Val Gln Val Ser Ile Asn Gln Leu Thr Ala Al #a Ile Tyr Asp Leu Leu                 645   #               650   #               655 Arg Leu His Ala Asn Ser Gly Arg Ser Pro Th #r Asp Cys Ala Gln Ser             660       #           665       #           670 Ser Lys Ser Val Lys Glu Ala Trp Thr Thr Th #r Glu Gln Leu Gln Phe         675           #       680           #       685 Thr Ile Phe Ala Ala His Gly Ile Ser Ser As #n Trp Val Ser Asn Tyr     690               #   695               #   700 Glu Lys Tyr Tyr Leu Ile Cys Ser Leu Ser Hi #s Asn Gly Lys Asp Leu 705                 7 #10                 7 #15                 7 #20 Phe Lys Pro Ile Gln Ser Lys Lys Val Gly Th #r Tyr Lys Asn Phe Phe                 725   #               730   #               735 Tyr Leu Ile Lys Trp Asp Glu Leu Ile Ile Ph #e Pro Ile Gln Ile Ser             740       #           745       #           750 Gln Leu Pro Leu Glu Ser Val Leu His Leu Th #r Leu Phe Gly Ile Leu         755           #       760           #       765 Asn Gln Ser Ser Gly Ser Ser Pro Asp Ser As #n Lys Gln Arg Lys Gly     770               #   775               #   780 Pro Glu Ala Leu Gly Lys Val Ser Leu Pro Le #u Cys Asp Phe Arg Arg 785                 7 #90                 7 #95                 8 #00 Phe Leu Thr Cys Gly Thr Lys Leu Leu Tyr Le #u Trp Thr Ser Ser His                 805   #               810   #               815 Thr Asn Ser Val Pro Gly Thr Val Thr Lys Ly #s Gly Tyr Val Met Glu             820       #           825       #           830 Arg Ile Val Leu Gln Val Asp Phe Pro Ser Pr #o Ala Phe Asp Ile Ile         835           #       840           #       845 Tyr Thr Thr Pro Gln Val Asp Arg Ser Ile Il #e Gln Gln His Asn Leu     850               #   855               #   860 Glu Thr Leu Glu Asn Asp Ile Lys Gly Lys Le #u Leu Asp Ile Leu His 865                 8 #70                 8 #75                 8 #80 Lys Asp Ser Ser Leu Gly Leu Ser Lys Glu As #p Lys Ala Phe Leu Trp                 885   #               890   #               895 Glu Lys Arg Tyr Tyr Cys Phe Lys His Pro As #n Cys Leu Pro Lys Ile             900       #           905       #           910 Leu Ala Ser Ala Pro Asn Trp Lys Trp Gly As #n Leu Ala Lys Thr Tyr         915           #       920           #       925 Ser Leu Leu His Gln Trp Pro Ala Leu Tyr Pr #o Leu Ile Ala Leu Glu     930               #   935               #   940 Leu Leu Asp Ser Lys Phe Ala Asp Gln Glu Va #l Arg Ser Leu Ala Val 945                 9 #50                 9 #55                 9 #60 Thr Trp Ile Glu Ala Ile Ser Asp Asp Glu Le #u Thr Asp Leu Leu Pro                 965   #               970   #               975 Gln Phe Val Gln Ala Leu Lys Tyr Glu Ile Ty #r Leu Asn Ser Ser Leu             980       #           985       #           990 Val Gln Phe Leu Leu Ser Arg Ala  Leu Gly  #Asn Ile Gln  Ile Ala His         995           #       1000           #       1005 Asn Leu  Tyr Trp Leu Leu Lys  Asp Ala L #eu His Asp  Val Gln Phe     1010              #    1015              #    1020 Ser Thr  Arg Tyr Glu His Val  Leu Gly A #la Leu Leu  Ser Val Gly     1025              #    1030              #    1035 Gly Lys  Arg Leu Arg Glu Glu  Leu Leu L #ys Gln Thr  Lys Leu Val     1040              #    1045              #    1050 Gln Leu  Leu Gly Gly Val Ala  Glu Lys V #al Arg Gln  Ala Ser Gly     1055              #    1060              #    1065 Ser Ala  Arg Gln Val Val Leu  Gln Arg S #er Met Glu  Arg Val Gln     1070              #    1075              #    1080 Ser Phe  Phe Gln Lys Asn Lys  Cys Arg L #eu Pro Leu  Lys Pro Ser     1085              #    1090              #    1095 Leu Val  Ala Lys Glu Leu Asn  Ile Lys S #er Cys Ser  Phe Phe Ser     1100              #    1105              #    1110 Ser Asn  Ala Val Pro Leu Lys  Val Thr M #et Val Asn  Ala Asp Pro     1115              #    1120              #    1125 Leu Gly  Glu Glu Ile Asn Val  Met Phe L #ys Val Gly  Glu Asp Leu     1130              #    1135              #    1140 Arg Gln  Asp Met Leu Ala Leu  Gln Met I #le Lys Ile  Met Asp Lys     1145              #    1150              #    1155 Ile Trp  Leu Lys Glu Gly Leu  Asp Leu A #rg Met Val  Ile Phe Lys     1160              #    1165              #    1170 Cys Leu  Ser Thr Gly Arg Asp  Arg Gly M #et Val Glu  Leu Val Pro     1175              #    1180              #    1185 Ala Ser  Asp Thr Leu Arg Lys  Ile Gln V #al Glu Tyr  Gly Val Thr     1190              #    1195              #    1200 Gly Ser  Phe Lys Asp Lys Pro  Leu Ala G #lu Trp Leu  Arg Lys Tyr     1205              #    1210              #    1215 Asn Pro  Ser Glu Glu Glu Tyr  Glu Lys A #la Ser Glu  Asn Phe Ile     1220              #    1225              #    1230 Tyr Ser  Cys Ala Gly Cys Cys  Val Ala T #hr Tyr Val  Leu Gly Ile     1235              #    1240              #    1245 Cys Asp  Arg His Asn Asp Asn  Ile Met L #eu Arg Ser  Thr Gly His     1250              #    1255              #    1260 Met Phe  His Ile Asp Phe Gly  Lys Phe L #eu Gly His  Ala Gln Met     1265              #    1270              #    1275 Phe Gly  Ser Phe Lys Arg Asp  Arg Ala P #ro Phe Val  Leu Thr Ser     1280              #    1285              #    1290 Asp Met  Ala Tyr Val Ile Asn  Gly Gly G #lu Lys Pro  Thr Ile Arg     1295              #    1300              #    1305 Phe Gln  Leu Phe Val Asp Leu  Cys Cys G #ln Ala Tyr  Asn Leu Ile     1310              #    1315              #    1320 Arg Lys  Gln Thr Asn Leu Phe  Leu Asn L #eu Leu Ser  Leu Met Ile     1325              #    1330              #    1335 Pro Ser  Gly Leu Pro Glu Leu  Thr Ser I #le Gln Asp  Leu Lys Tyr     1340              #    1345              #    1350 Val Arg  Asp Ala Leu Gln Pro  Gln Thr T #hr Asp Ala  Glu Ala Thr     1355              #    1360              #    1365 Ile Phe  Phe Thr Arg Leu Ile  Glu Ser S #er Leu Gly  Ser Ile Ala     1370              #    1375              #    1380 Thr Lys  Phe Asn Phe Phe Ile  His Asn L #eu Ala Gln  Leu Arg Phe     1385              #    1390              #    1395 Ser Gly  Leu Pro Ser Asn Asp  Glu Pro I #le Leu Ser  Phe Ser Pro     1400              #    1405              #    1410 Lys Thr  Tyr Ser Phe Arg Gln  Asp Gly A #rg Ile Lys  Glu Val Ser     1415              #    1420              #    1425 Val Phe  Thr Tyr His Lys Lys  Tyr Asn P #ro Asp Lys  His Tyr Ile     1430              #    1435              #    1440 Tyr Val  Val Arg Ile Leu Trp  Glu Gly G #ln Ile Glu  Pro Ser Phe     1445              #    1450              #    1455 Val Phe  Arg Thr Phe Val Glu  Phe Gln G #lu Leu His  Asn Lys Leu     1460              #    1465              #    1470 Ser Ile  Ile Phe Pro Leu Trp  Lys Leu P #ro Gly Phe  Pro Asn Arg     1475              #    1480              #    1485 Met Val  Leu Gly Arg Thr His  Ile Lys A #sp Val Ala  Ala Lys Arg     1490              #    1495              #    1500 Lys Ile  Glu Leu Asn Ser Tyr  Leu Gln S #er Leu Met  Asn Ala Ser     1505              #    1510              #    1515 Thr Asp  Val Ala Glu Cys Asp  Leu Val C #ys Thr Phe  Phe His Pro     1520              #    1525              #    1530 Leu Leu  Arg Asp Glu Lys Ala  Glu Gly I #le Ala Arg  Ser Ala Asp     1535              #    1540              #    1545 Ala Gly  Ser Phe Ser Pro Thr  Pro Gly G #ln Ile Gly  Gly Ala Val     1550              #    1555              #    1560 Lys Leu  Ser Ile Ser Tyr Arg  Asn Gly T #hr Leu Phe  Ile Met Val     1565              #    1570              #    1575 Met His  Ile Lys Asp Leu Val  Thr Glu A #sp Gly Ala  Asp Pro Asn     1580              #    1585              #    1590 Pro Tyr  Val Lys Thr Tyr Leu  Leu Pro A #sp Asn His  Lys Thr Ser     1595              #    1600              #    1605 Lys Arg  Lys Thr Lys Ile Ser  Arg Lys T #hr Arg Asn  Pro Thr Phe     1610              #    1615              #    1620 Asn Glu  Met Leu Val Tyr Ser  Gly Tyr S #er Lys Glu  Thr Leu Arg     1625              #    1630              #    1635 Gln Arg  Glu Leu Gln Leu Ser  Val Leu S #er Ala Glu  Ser Leu Arg     1640              #    1645              #    1650 Glu Asn  Phe Phe Leu Gly Gly  Val Thr L #eu Pro Leu  Lys Asp Phe     1655              #    1660              #    1665 Asn Leu  Ser Lys Glu Thr Val  Lys Trp T #yr Gln Leu  Thr Ala Ala     1670              #    1675              #    1680 Thr Tyr  Leu     1685 <210> SEQ ID NO 3 <211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: primer <400> SEQUENCE: 3 ctcttcctca tggtctaata cctccac           #                   #             27 <210> SEQ ID NO 4 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: primer <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (3)..(3) <223> OTHER INFORMATION: n can be any nucleotid #e <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (15)..(15) <223> OTHER INFORMATION: n can be any nucleotid #e <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (6)..(6) <223> OTHER INFORMATION: n can be either T o #r C <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (9)..(9) <223> OTHER INFORMATION: n can be T or C <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (10)..(10) <223> OTHER INFORMATION: n can be either T o #r C. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (12)..(12) <223> OTHER INFORMATION: n can be either A o #r G. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (18)..(18) <223> OTHER INFORMATION: n can be either A o #r G. <400> SEQUENCE: 4 ggngangann tncgncanga             #                   #                   # 20 <210> SEQ ID NO 5 <211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: primer <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (16)..(16) <223> OTHER INFORMATION: n can be either A,  #G, or T. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (7)..(7) <223> OTHER INFORMATION: n is an I. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (1)..(1) <223> OTHER INFORMATION: n can be A or G. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (4)..(4) <223> OTHER INFORMATION: n can be A or G.. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (10)..(10) <223> OTHER INFORMATION: n can be A or G. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (13)..(13) <223> OTHER INFORMATION: n can be A or G. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (19)..(19) <223> OTHER INFORMATION: n can be A or G. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (22)..(22) <223> OTHER INFORMATION: n can be A or G. <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (25)..(25) <223> OTHER INFORMATION: n can be A or G. <400> SEQUENCE: 5 naantgnccn aantcnatnt gntgnaa           #                   #             27 <210> SEQ ID NO 6 <211> LENGTH: 27 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: primer <400> SEQUENCE: 6 tatctccaaa tcagtccttg ctttccc           #                   #             27 <210> SEQ ID NO 7 <211> LENGTH: 147 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <400> SEQUENCE: 7 Met Pro Thr Phe Pro Ser Thr Glu Ser Val Ty #r Leu Arg Leu Pro Gly 1               5    #                10   #                15 Gln Ser Pro Tyr Phe Ser Tyr Pro Leu Thr Pr #o Ala Thr Pro Phe His             20       #            25       #            30 Pro Gln Gly Ser Leu Pro Val Tyr Arg Pro Le #u Val Ser Pro Asp Met         35           #        40           #        45 Ala Lys Leu Phe Glu Lys Ile Ala Ser Thr Se #r Glu Phe Leu Lys Asn     50               #    55               #    60 Gly Lys Ala Arg Thr Asp Leu Glu Ile Ala As #n Ser Lys Ala Ser Val 65                   #70                   #75                   #80 Cys Asn Leu Gln Ile Ser Pro Lys Ser Glu As #p Ile Asn Lys Phe Asp                 85   #                90   #                95 Trp Leu Asp Leu Asp Pro Leu Ser Lys Pro Ly #s Val Asp Tyr Val Glu             100       #           105       #           110 Val Leu Glu His Glu Glu Glu Lys Lys Asp Pr #o Val Leu Leu Ala Glu         115           #       120           #       125 Asp Pro Trp Asp Ala Val Leu Leu Glu Glu Ar #g Ser Pro Ser Cys His     130               #   135               #   140 Leu Glu Arg 145 <210> SEQ ID NO 8 <211> LENGTH: 294 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <400> SEQUENCE: 8 Met Ala Gln Ile Ser Asn Asn Ser Glu Phe Ly #s Gln Cys Ser Ser Ser 1               5    #                10   #                15 His Pro Glu Pro Ile Arg Thr Lys Asp Val As #n Lys Ala Glu Ala Leu             20       #            25       #            30 Gln Met Glu Ala Glu Ala Leu Ala Lys Leu Gl #n Lys Asp Arg Gln Met         35           #        40           #        45 Thr Asp Ser Pro Arg Gly Phe Glu Leu Ser Se #r Ser Thr Arg Gln Arg     50               #    55               #    60 Thr Gln Gly Phe Asn Lys Gln Asp Tyr Asp Le #u Met Val Phe Pro Glu 65                   #70                   #75                   #80 Leu Asp Ser Gln Lys Arg Ala Val Asp Ile As #p Val Glu Lys Leu Thr                 85   #                90   #                95 Gln Ala Glu Leu Glu Lys Ile Leu Leu Asp As #p Asn Phe Glu Thr Arg             100       #           105       #           110 Lys Pro Pro Ala Leu Pro Val Thr Pro Val Le #u Ser Pro Ser Phe Ser         115           #       120           #       125 Thr Gln Leu Tyr Leu Arg Pro Ser Gly Gln Ar #g Gly Gln Trp Pro Pro     130               #   135               #   140 Gly Leu Cys Gly Pro Ser Thr Tyr Thr Leu Pr #o Ser Thr Tyr Pro Ser 145                 1 #50                 1 #55                 1 #60 Ala Tyr Ser Lys Gln Ala Thr Phe Gln Asn Gl #y Phe Ser Pro Arg Met                 165   #               170   #               175 Pro Thr Phe Pro Ser Thr Glu Ser Val Tyr Le #u Arg Leu Pro Gly Gln             180       #           185       #           190 Ser Pro Tyr Phe Ser Tyr Pro Leu Thr Pro Al #a Thr Pro Phe His Pro         195           #       200           #       205 Gln Gly Ser Leu Pro Val Tyr Arg Pro Leu Va #l Ser Pro Asp Met Ala     210               #   215               #   220 Lys Leu Phe Glu Lys Ile Ala Ser Thr Ser Gl #u Phe Leu Lys Asn Gly 225                 2 #30                 2 #35                 2 #40 Lys Ala Arg Thr Asp Leu Glu Ile Ala Asn Se #r Lys Ala Ser Val Cys                 245   #               250   #               255 Asn Leu Gln Ile Ser Pro Lys Ser Glu Asp Il #e Asn Lys Phe Asp Trp             260       #           265       #           270 Leu Asp Leu Asp Pro Trp Asp Ala Val Leu Le #u Glu Glu Arg Ser Pro         275           #       280           #       285 Ser Cys His Leu Glu Arg     290 <210> SEQ ID NO 9 <211> LENGTH: 7 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <220> FEATURE: <221> NAME/KEY: MISC_FEATURE <222> LOCATION: (7)..(7) <223> OTHER INFORMATION: Xaa can be Asp or G #lu <400> SEQUENCE: 9 Gly Asp Asp Leu Arg Gly Xaa 1               5 <210> SEQ ID NO 10 <211> LENGTH: 8 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <400> SEQUENCE: 10 Phe His Ile Asp Phe Gly His Phe 1               5 <210> SEQ ID NO 11 <211> LENGTH: 6 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <400> SEQUENCE: 11 Pro Pro Leu Pro Pro Arg 1               5 

What is claimed is:
 1. A method for identifying a ligand which modulates kinase activity of a class II PI 3 kinase, said method comprising the steps of: i) exposing a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or a ligand-binding fragment thereof to at least one ligand; and ii) determining the binding activity of said at least one ligand with respect to said polypeptide.
 2. A method according to claim 1, wherein said polypeptide comprises amino acids 1-337 of SEQ ID NO:
 2. 3. A method according to claim 1, wherein said polypeptide comprises amino acids 1-176 of SEQ ID NO:
 2. 4. A method according to claim 1, wherein said polypeptide is expressed by a cell-line which has been transformed or transfected with a nucleic acid comprising a nucleic acid sequence which hybridizes to SEQ ID NO:1 in 0.5 M sodium phsophate, pH 7.2, 7% SDS, 1 mM EDTA at 65° C. and remains bound after two washing steps with 0.5 x SSC and 0.1% SDS for 20 minutes at 60° C.
 5. A method according to claim 4, wherein said cell line is a prokaryotic cell line.
 6. A method according to claim 4, wherein said cell line is a eukaryotic cell line.
 7. A method according to claim 6, wherein said cell-line is a mammalian cell line.
 8. A method according to claim 7, wherein said mammalian cell line is a kidney cell line.
 9. A method according to claim 4, wherein said nucleic acid is a recombinant vector which comprises: i) a nucleotide sequence which encodes a class II PI3 kinase having the amino acid sequence of SEQ ID NO:2 or a ligand-binding fragment thereof; and ii) a DNA promoter element which is functionally linked to said nucleotide sequence.
 10. A method according to claim 1, wherein said ligand is a peptide or a polypeptide.
 11. A method according to claim 1, wherein said ligand binds the amino-terminal domain of SEQ ID NO:2.
 12. A method according to claim 11, wherein said amino-terminal domain comprises amino acids 1-337 of SEQ ID NO:2.
 13. A method according to claim 11, wherein said amino-terminal domain comprises amino acids 1-176 of SEQ ID NO:2.
 14. A method according to claim 1, wherein said ligand is a class II PI 3 kinase antagonist.
 15. A method according to claim 1, wherein said ligand is a class II PI 3 kinase agonist.
 16. A method according to claim 1, wherein said binding activity modulates the kinase activity of a polypeptide comprising SEQ ID NO:2.
 17. A method according to claim 16, wherein the modulation affects addition of phosphate to a substrate of said enzyme.
 18. A method according to claim 17, wherein said substrate is selected from the group consisting of inositol and PtdIns.
 19. A method according to claim 17, wherein said substrate is PtdIns or PtdIns(4)P.
 20. A method according to claim 16, wherein the modulation of kinase activity by said ligand results in an inhibition of cell division.
 21. A method according to claim 20, wherein the cell is a neutrophil.
 22. A method according to claim 16, wherein the modulation of kinase activity by said ligand results in a stimulation of cell division.
 23. A method according to claim 22, wherein the cell is a neutrophil.
 24. A method according to claim 1, further comprising: preparing a computer aided reconstruction of the 3-dimensional structure of the polypeptide comprising the amino acid sequence of SEQ ID NO:2; and comparing the 3-dimensional structure to the structure of at least one candidate ligand to predict the interaction of the candidate ligand with said polypeptide.
 25. A method according to claim 24, wherein said polypeptide comprises amino acids 1-337 of SEQ ID NO:2.
 26. A method according to claim 24, wherein said polypeptide comprises amino acids 1-176 of SEQ ID NO:2. 